Germinação de sementes de Aspidosperma subincanumMart. ex A. DC em diferentes temperaturas / Germination of Aspidosperma subincanum Mart. ex A. DC seeds at different temperatures

RESUMO : Aspidosperma subincanum é uma espécie arbórea utilizada em projetos de recuperação de áreas degradadas e como planta medicinal. Levando-se em consideração a importância da espécie, o presente trabalho teve como objetivo definir a temperatura ideal para a germinação de suas sementes. O material vegetal foi coletado na região do Pantanal de Miranda, Mato Grosso do Sul. As sementes foram colocadas em caixas plásticas transparentes em câmara de germinação e submetidas às temperaturas constantes de 20, 25, 30 e 35°C e alternadas de 20-30 e 25-35°C. O delineamento experimental foi inteiramente casualizado, com quatro repetições por tratamento, sendo as médias comparadas pelo teste de Tukey a 5%. A temperatura ideal para a germinação de Aspidosperma subincanum foi a 25°C, com o maior índice de velocidade de germinação, 18,8. Temperaturas mais elevadas (30 e 35°C) causaram a morte dos embriões

ABSTRACT Germination of Aspidosperma subincanum Mart. ex A. DC seeds at different temperatures. The Aspidosperma subincanumem is a tree species used in the recovery of degraded areas projects and as a medicinal plant. Taking into account the importance of the species, the current study aimed to determine which would be the ideal temperature for the germination of their seeds. The plant material was collected in the Pantanal of Miranda, Mato Grosso do Sul. The seeds were placed in transparent boxes in germination chambers and submitted to constant temperatures of, 20, 25, 30 and 35°C and alternated at 20-30 and 25- 35°C, with four replicates per treatment. The statistical design was a completely randomized one. The averages were compared by Tukey test at 5%. The ideal temperature for germination of Aspidosperma subincanum was 25°C. The high temperatures (30 and 35°C) have caused the death of embryos

In vitro antimalarial activity of six Aspidosperma species from the state of Minas Gerais (Brazil).

Ethnomedicinal informations point to some Aspidosperma species (Apocynaceae) as antimalarial plants in Brazil and have motivated the evaluation of six species which were collected in the state of Minas Gerais: A. cylindrocarpon Müll. Arg., A. parvifolium A. DC., A. olivaceum Müll. Arg., A. ramiflorum Müll. Arg., A. spruceanum Benth. ex Müll. Arg. and A. tomentosum Mart.. A total of 23 extracts of different plant parts in different solvents were assayed in vitro against chloroquine-resistant (W2) and chloroquine-sensitive (3D7) strains of Plasmodium falciparum. All the extracts were shown to be active with IC50 values in the range of 5.0 ± 0 2.8 µg/mL to 65.0 ± 4.2 µg/mL. TLC profile of the extracts revealed the presence of alkaloids in the six species assayed. These results seem to confirm the popular use of Aspidosperma species to treat human malaria in Brazil and seem point to alkaloids as the putative active compounds of the assayed species.

Microsatellite markers for Aspidosperma polyneuron (Apocynaceae), an endangered tropical tree species.

PREMISE OF THE STUDY: This study was designed to develop and characterize a preliminary set of nuclear microsatellite markers for Aspidosperma polyneuron, an endangered tropical tree species. METHODS AND RESULTS: Leaf samples were collected from a group of mature A. polyneuron trees in a large area along the Pardo and Mogi-Guaçu river basins in São Paulo State in the southeastern region of Brazil. Twenty-five simple sequence repeat (SSR) markers were developed and 16 of 30 samples had polymorphic loci. The number of alleles per locus ranged from two to 17 (average = 7.06). A high diversity (H(e) = 0.65) and parentage exclusion probability for the first (0.9995) and second (0.9999) parents were measured. CONCLUSIONS: These 16 polymorphic markers suggest a high potential for genetic diversity, gene flow, and mating system analyses in A. polyneuron.